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If the collagen is in poor preservation condition, the lab will contact you for discussion before proceeding further.If the collagen passes visual inspection, sodium hydroxide (Na OH) is applied to remove humic and exogenous organic contaminants.Bones that have been drilled or powdered prior to submission must be cleaned of any adhering or invasive contamination prior to the drilling or powdering.This many times requires both physical abrasion of the surface and chemical treatments.The bone sample is first tested for friability (“softness”).Very soft bone material is an indication of the potential absence of the collagen fraction (basal bone protein acting as a “reinforcing agent” within the crystalline apatite structure).However, the material will be listed as “organics” in the final report instead of “bone collagen.” This is due to the requirement by our ISO/IEC 17005 accreditation that if we did not perform the chemical pretreatments and/or extractions, we cannot specifically testify as to the material that was dated because certain steps affecting the sample quality have been outside of our control and scope of our accreditation.Do not place extracted collagen directly into Ziplock bags.
Thus, it’s possible that the bones have very limited collagen.
When requesting for an estimate/quotation, please provide the currency, number of samples, service requested/turnaround time (AMS standard or priority), type (non-heated, fully charred or cremated bones) and the billing address.
Bones – Good cortical bone is best from the larger bones of the body (femur, tibia, upper arm bone, jaw, skull plate and sometimes the ribs).
– Fees are inclusive of d13C measurements, d15N measurements (except cremated bones), quality assurance reports, calendar calibration when applicable, and 24/7 web access to past results and pending analyses.
Additional fee is charged for collagen or bone carbonate extraction.